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Part:BBa_K2996506

Designed by: Shifeng Jiang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-03)


Combination of sgRNA production module and target-DBD binding site

This part is used as an sgRNA production module and a detection site.It contains part BBa_K2996504,which is the binding part for both the NdCas9 and DBD protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 287
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 199
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 287
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 287
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None